| Download | - View final version: Zika virus crosses an in vitro human blood brain barrier model (PDF, 1.4 MiB)
|
|---|
| DOI | Resolve DOI: https://doi.org/10.1186/s12987-018-0100-y |
|---|
| Author | Search for: Alimonti, Judie B.1; Search for: Ribecco-Lutkiewicz, Maria1; Search for: Sodja, Caroline1; Search for: Jezierski, Anna1; Search for: Stanimirovic, Danica B.1; Search for: Liu, Qing; Search for: Haqqani, Arsalan S.1; Search for: Conlan, Wayne1; Search for: Bani-Yaghoub, Mahmud |
|---|
| Affiliation | - National Research Council of Canada. Human Health Therapeutics
|
|---|
| Format | Text, Article |
|---|
| Subject | Zika virus; brain endothelial cells; blood–brain barrier model; iPSC; AXL; neural progenitors |
|---|
| Abstract | Zika virus (ZIKV) is a flavivirus that is highly neurotropic causing congenital abnormalities and neurological damage to the central nervous systems (CNS). In this study, we used a human induced pluripotent stem cell (iPSC)-derived blood brain barrier (BBB) model to demonstrate that ZIKV can infect brain endothelial cells (i-BECs) without compromising the BBB barrier integrity or permeability. Although no disruption to the BBB was observed post-infection, ZIKV particles were released on the abluminal side of the BBB model and infected underlying iPSC-derived neural progenitor cells (i-NPs). AXL, a putative ZIKV cellular entry receptor, was also highly expressed in ZIKV-susceptible i-BEC and i-NPs. This iPSC-derived BBB model can help elucidate the mechanism by which ZIKV can infect BECs, cross the BBB and gain access to the CNS. |
|---|
| Publication date | 2018-05-15 |
|---|
| Publisher | BMC |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NPARC number | 23004144 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 4bc86a85-4326-4aed-8044-176c02d7047c |
|---|
| Record created | 2018-10-02 |
|---|
| Record modified | 2020-05-30 |
|---|
