| Download | - View final version: Cross-validation of ELISA and a portable surface plasmon resonance instrument for IgG antibody serology with SARS-CoV-2 positive individuals (PDF, 1.5 MiB)
- View supplementary information: Cross-validation of ELISA and a portable surface plasmon resonance instrument for IgG antibody serology with SARS-CoV-2 positive individuals (PDF, 1.5 MiB)
|
|---|
| DOI | Resolve DOI: https://doi.org/10.1039/D1AN00893E |
|---|
| Author | Search for: Djaileb, Abdelhadi; Search for: Hojjat Jodaylami, Maryam; Search for: Coutu, Julien; Search for: Ricard, Pierre; Search for: Lamarre, Mathieu; Search for: Rochet, Léa; Search for: Cellier-Goetghebeur, Stella; Search for: Macaulay, Devin; Search for: Charron, Benjamin; Search for: Lavallée, Étienne; Search for: Thibault, Vincent; Search for: Stevenson, Keisean; Search for: Forest, Simon; Search for: Live, Ludovic S.; Search for: Abonnenc, Nanouk; Search for: Guedon, Anthony; Search for: Quessy, Patrik; Search for: Lemay, Jean-François; Search for: Farnós, Omar; Search for: Kamen, Amine; Search for: Stuible, Matthew; Search for: Gervais, Christian1ORCID identifier: https://orcid.org/0000-0002-0519-3054; Search for: Durocher, Yves1; Search for: Cholette, François; Search for: Mesa, Christine; Search for: Kim, John; Search for: Cayer, Marie-Pierre; Search for: de Grandmont, Marie-Joëlle; Search for: Brouard, Danny; Search for: Trottier, Sylvie; Search for: Boudreau, DenisORCID identifier: https://orcid.org/0000-0001-5152-2464; Search for: Pelletier, Joelle N.; Search for: Masson, Jean-FrancoisORCID identifier: https://orcid.org/0000-0002-0101-0468 |
|---|
| Affiliation | - National Research Council of Canada. Human Health Therapeutics
|
|---|
| Format | Text, Article |
|---|
| Abstract | We report on the development of surface plasmon resonance (SPR) sensors and matching ELISAs for the detection of nucleocapsid and spike antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma and dried blood spots (DBS). When exposed to SARS-CoV-2 or a vaccine against SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the fraction of the population potentially immunized against SARS-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with various sources of SARS-CoV-2 recombinant proteins expressed in different cell lines detected human anti-SARS-CoV-2 IgG antibodies in clinical samples. Nucleocapsid expressed in different cell lines did not significantly change the sensitivity of the assays, whereas the use of a CHO cell line to express spike ectodomain led to excellent performance. This bioassay was performed on a portable SPR instrument capable of measuring 4 biological samples within 30 minutes of sample/sensor contact and the chip could be regenerated at least 9 times. Multi-site validation was then performed with in-house and commercial ELISA, which revealed excellent cross-correlations with Pearson's coefficients exceeding 0.85 in all cases, for measurements in DBS and plasma. This strategy paves the way to point-of-care and rapid testing for antibodies in the context of viral infection and vaccine efficacy monitoring. |
|---|
| Publication date | 2021-07-01 |
|---|
| Publisher | Royal Society of Chemistry |
|---|
| Licence | |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 4efd885e-c789-4019-adfc-b6ae806d5302 |
|---|
| Record created | 2022-10-05 |
|---|
| Record modified | 2022-10-05 |
|---|
