| Téléchargement | - Voir la version finale : Cross-validation of ELISA and a portable surface plasmon resonance instrument for IgG antibody serology with SARS-CoV-2 positive individuals (PDF, 1.5 Mio)
- Voir les données supplémentaires : Cross-validation of ELISA and a portable surface plasmon resonance instrument for IgG antibody serology with SARS-CoV-2 positive individuals (PDF, 1.5 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1039/D1AN00893E |
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| Auteur | Rechercher : Djaileb, Abdelhadi; Rechercher : Hojjat Jodaylami, Maryam; Rechercher : Coutu, Julien; Rechercher : Ricard, Pierre; Rechercher : Lamarre, Mathieu; Rechercher : Rochet, Léa; Rechercher : Cellier-Goetghebeur, Stella; Rechercher : Macaulay, Devin; Rechercher : Charron, Benjamin; Rechercher : Lavallée, Étienne; Rechercher : Thibault, Vincent; Rechercher : Stevenson, Keisean; Rechercher : Forest, Simon; Rechercher : Live, Ludovic S.; Rechercher : Abonnenc, Nanouk; Rechercher : Guedon, Anthony; Rechercher : Quessy, Patrik; Rechercher : Lemay, Jean-François; Rechercher : Farnós, Omar; Rechercher : Kamen, Amine; Rechercher : Stuible, Matthew; Rechercher : Gervais, Christian1Identifiant ORCID : https://orcid.org/0000-0002-0519-3054; Rechercher : Durocher, Yves1; Rechercher : Cholette, François; Rechercher : Mesa, Christine; Rechercher : Kim, John; Rechercher : Cayer, Marie-Pierre; Rechercher : de Grandmont, Marie-Joëlle; Rechercher : Brouard, Danny; Rechercher : Trottier, Sylvie; Rechercher : Boudreau, DenisIdentifiant ORCID : https://orcid.org/0000-0001-5152-2464; Rechercher : Pelletier, Joelle N.; Rechercher : Masson, Jean-FrancoisIdentifiant ORCID : https://orcid.org/0000-0002-0101-0468 |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Résumé | We report on the development of surface plasmon resonance (SPR) sensors and matching ELISAs for the detection of nucleocapsid and spike antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma and dried blood spots (DBS). When exposed to SARS-CoV-2 or a vaccine against SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the fraction of the population potentially immunized against SARS-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with various sources of SARS-CoV-2 recombinant proteins expressed in different cell lines detected human anti-SARS-CoV-2 IgG antibodies in clinical samples. Nucleocapsid expressed in different cell lines did not significantly change the sensitivity of the assays, whereas the use of a CHO cell line to express spike ectodomain led to excellent performance. This bioassay was performed on a portable SPR instrument capable of measuring 4 biological samples within 30 minutes of sample/sensor contact and the chip could be regenerated at least 9 times. Multi-site validation was then performed with in-house and commercial ELISA, which revealed excellent cross-correlations with Pearson's coefficients exceeding 0.85 in all cases, for measurements in DBS and plasma. This strategy paves the way to point-of-care and rapid testing for antibodies in the context of viral infection and vaccine efficacy monitoring. |
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| Date de publication | 2021-07-01 |
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| Maison d’édition | Royal Society of Chemistry |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 4efd885e-c789-4019-adfc-b6ae806d5302 |
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| Enregistrement créé | 2022-10-05 |
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| Enregistrement modifié | 2022-10-05 |
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