| Download | - View accepted manuscript: Activation of Nanoparticles by Biosorption for E. coli Detection in Milk and Apple Juice (PDF, 933 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s12010-009-8709-6 |
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| Author | Search for: Naja, Ghinwa1; Search for: Bouvrette, Pierre1; Search for: Champagne, Julie1; Search for: Brousseau, Roland1; Search for: Luong, John H. T.1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | contaminated milk and apple juice; nanoparticles; detection limit; bacterial capture; biosorption; Escherichia coli |
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| Abstract | Two types of silver nanoparticles were activated by specific sorption of biomolecules for the detection of Escherichia coli. The capture of this bacterium was performed using polyclonal antibodies (anti-E. coli) biosorbed onto nanospheres or nanorice through a protein-A layer. The bacterial detection was achieved using surface enhancement Raman scattering in order to compare the performance of these two nanoparticles. The activated silver nanospheres showed a better performance mainly due to the dimension of these nanoparticles. The detection limit has been established using the automated Raman mapping system. The technique was capable of detecting 103 cells/mL in milk and apple juice without any pre-enrichment. With an overall assay time less than 1�h, the process could be easily adapted to detect other pathogens by selecting the pertinent antibody. Furthermore, PCR was used for the DNA verification to assess whether the selected bacterial strain was identical before and after detection. © 2009 Humana Press |
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| Publication date | 2010 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 49991 |
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| NPARC number | 15651320 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 4f1d88d0-6565-41cf-9789-184d4de6de95 |
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| Record created | 2010-11-05 |
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| Record modified | 2022-11-18 |
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