| Author | Search for: Altman, Eleonora; Search for: Brisson, J.; Search for: Gagne, S.; Search for: Kolbe, J.; Search for: Messner, P.; Search for: Sleytr, U. |
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| Format | Text, Article |
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| Subject | acid; bacterial; bacterial outer membrane proteins; band; Canada; carbohydrate; carbohydrate sequence; cell; cell wall; chain; chemical; chemistry; chromatography, gel; clostridium; deglycosylation; digestion; electrophoresis; filtration; freeze etching; glycan chain; glycopeptide; glycoprotein; homonuclear; isolation & purification; magnetic; magnetic resonance spectroscopy; mass fragmentography; membrane; membrane glycoproteins; membrane proteins; mesylates; methylation; models, molecular; molecular; molecular sequence data; nuclear; nuclear magnetic resonance; polysaccharide; protein; resonance; sodium; structure; subunit; Support, Non-U. S. Gov't; surface; ultrastructure |
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| Abstract | The thermophilic eubacterium Clostridium thermohydrosulfuricum L77-66 is covered by a crystalline surface layer composed of identical glycoprotein subunits which are arranged in a hexagonal lattice with centre-to-centre spacings of approx. 14.3 nm. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of cell wall preparations showed the presence of several broadened, carbohydrate-containing bands in a molecular mass range of 90 to 200 kDa. A total carbohydrate content of approx. 14% was determined in the purified surface layer glycoprotein. Chemical deglycosylation of this material by trifluoromethanesulfonic acid resulted in the disappearance of the complex banding pattern. Only a single band with a molecular mass of 82 kDa remained visible upon Coomassie staining. After proteolytic digestion of the surface layer glycoprotein a single glycopeptide fraction with an apparent molecular mass of approx. 25 kDa was obtained by gel filtration. Composition analysis, methylation, periodate oxidation and a combination of homonuclear and 1H-detected heteronuclear shift-correlated nuclear magnetic resonance experiments established the following structure for the glycan chain of the surface layer glycoprotein |
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| Publication date | 1992-07-21 |
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| In | |
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| Language | English |
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| NRC number | ALTMAN1992 |
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| NPARC number | 9367195 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 515149bf-17c0-450b-833e-117d89b7d122 |
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| Record created | 2009-07-10 |
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| Record modified | 2020-04-24 |
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