This report describes seakeeping experiments carried out on the 45 ft. (13.72 m) fishing vessel CCGA Nautical Twilight off St. John's, NL November 20, 2003. Collaborators involved in the fishing vessel sea trials include the Institute for Ocean Technology (IOT), Memorial University of Newfoundland (MUN), Oceanic Consulting Corp. (OCC), Canadian Coast Guard (CCG), the Offshore Safety and Survival Centre (OSSC) of the Marine Institute and SafetyNet - a Community Research Alliance on Health and Safety in Marine and Coastal Work. Primary financial support for the project is provided from federal funding sources including the Search & Rescue (SAR), New Initiatives Fund (NIF) and the Canadian Institutes of Health and Research (CIHR) in addition to significant in-kind contributions from the many participants. The objective of the project is to acquire quality full scale motions data on fishing vessels to validate physical model methodology as well as numerical simulation models under development. Eventually, tools will be developed and validated to evaluate the number of Motion Induced Interrupts (MIIs), induced by sudden ship motions, and their impact on crew accidents to develop criteria to reduce MIIs. This document describes the CCGA Nautical Twilight, the trials instrumentation package, data acquisition system, test program, data analysis procedure and presents the results. Unfortunately there was a failure of the directional wave buoy deployed during the trial and no valid wave information data was acquired. Thus although a series of short runs were completed for the benefit of research underway by MUN Kinesiology staff, this trial cannot be considered a success and this report merely documents effort carried out. There may be an opportunity to repeat the trial on the "Nautical Twilight" or equivalent vessel in the future.