Different iron-chelating properties of pyochelin diastereoisomers revealed by LC/MS

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/s00216-006-0443-0
AuthorSearch for: 1; Search for: 1
Name affiliation
  1. National Research Council of Canada. NRC Institute for Marine Biosciences
FormatText, Article
Subjectpyochelin; pseudomonas siderophore; iron complex; electrospray ionization; liquid chromatography/mass spectrometry
Abstract
Pyochelin is a siderophore and virulence factor common to Burkholderia cepacia and several Pseudomonas strains. It is isolated from bacterial media as a mixture of two epimers, which readily equilibrate in most solvents. Experiments based on high-performance liquid chromatography/electrospray ionization mass spectrometry are reported here, allowing the investigation of the different Fe(III)-chelating properties of pyochelin diastereomers in solution without the need for labourious isolation. It is demonstrated in this study that only one of the two pyochelin diastereomers is able to chelate Fe(III); no Fe(III) complexes of the other diastereomer could be detected. The Fe(III)-pyochelin complex exhibited a 1:1 metal-to-siderophore ratio and no evidence for other stoichiometries was found.
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Terms of use
  • Copyright © 2007, Blackwell Publishing Ltd.
In
  • Analytical and Bioanalytical Chemistry 385, no. 3 (May 6, 2006): 606–611.
LanguageEnglish
Peer reviewedYes
NRC numberNRCC 55867
1527
NPARC number3538405
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Record identifier6568105e-258d-4fcf-bc00-029e7464c65f
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