Download | - View accepted manuscript: ABA 9'-hydroxylation is catalyzed by CYP707A in Arabidopsis (PDF, 973 KiB)
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DOI | Resolve DOI: https://doi.org/10.1016/j.phytochem.2011.02.004 |
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Author | Search for: Okamoto, Masanori; Search for: Kushiro, Tetsuo; Search for: Jikumaru, Yusuke; Search for: Abrams, Suzanne R.1; Search for: Kamiya, Yuji; Search for: Seki, Motoaki; Search for: Nambara, Eiji |
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Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
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Format | Text, Article |
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Subject | Arabidopsis; abscisic acid; catabolism; P450; CYP707A; neophaseic acid; phaseic acid |
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Abstract | Abscisic acid (ABA) catabolism is important for regulating endogenous ABA levels. Neophaseic acid (neoPA) is one of the ABA catabolites produced via ABA 9'-hydroxylation, but the gene encoding ABA 9'-hydroxylase has not been identified. We found that endogenous neoPA levels reduced in loss-of-function mutants defective in ABA 8'-hydroxylase (CYP707A) genes. In addition, levels of both neoPA and PA reduced similarly when wild-type plants were treated with uniconazole-P, a P450 inhibitor. Furthermore, in vitro enzyme assay using microsomal fraction from yeast expressing CYP707A showed that all four Arabidopsis CYP707As contained both ABA 8'- and 9'-hydroxylase activities, although ABA 9'-hydroxylase activity is minor. These results demonstrate that ABA 9'- hydroxylation is catalyzed by CYP707A as a side reaction in Arabidopsis. |
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Publication date | 2011-03-15 |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NRC number | NRCC 50177 |
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NPARC number | 18856993 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 6fe8d976-cdf5-4451-b340-d0da5001aaaa |
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Record created | 2012-03-30 |
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Record modified | 2020-04-21 |
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