| Téléchargement | - Voir le manuscrit accepté : ABA 9'-hydroxylation is catalyzed by CYP707A in Arabidopsis (PDF, 973 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1016/j.phytochem.2011.02.004 |
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| Auteur | Rechercher : Okamoto, Masanori; Rechercher : Kushiro, Tetsuo; Rechercher : Jikumaru, Yusuke; Rechercher : Abrams, Suzanne R.1; Rechercher : Kamiya, Yuji; Rechercher : Seki, Motoaki; Rechercher : Nambara, Eiji |
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| Affiliation | - Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
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| Format | Texte, Article |
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| Sujet | Arabidopsis; abscisic acid; catabolism; P450; CYP707A; neophaseic acid; phaseic acid |
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| Résumé | Abscisic acid (ABA) catabolism is important for regulating endogenous ABA levels. Neophaseic acid (neoPA) is one of the ABA catabolites produced via ABA 9'-hydroxylation, but the gene encoding ABA 9'-hydroxylase has not been identified. We found that endogenous neoPA levels reduced in loss-of-function mutants defective in ABA 8'-hydroxylase (CYP707A) genes. In addition, levels of both neoPA and PA reduced similarly when wild-type plants were treated with uniconazole-P, a P450 inhibitor. Furthermore, in vitro enzyme assay using microsomal fraction from yeast expressing CYP707A showed that all four Arabidopsis CYP707As contained both ABA 8'- and 9'-hydroxylase activities, although ABA 9'-hydroxylase activity is minor. These results demonstrate that ABA 9'- hydroxylation is catalyzed by CYP707A as a side reaction in Arabidopsis. |
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| Date de publication | 2011-03-15 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 50177 |
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| Numéro NPARC | 18856993 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 6fe8d976-cdf5-4451-b340-d0da5001aaaa |
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| Enregistrement créé | 2012-03-30 |
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| Enregistrement modifié | 2020-04-21 |
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