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The effect of exogenous DNA on the nodulation of a non-nodulating line of Pisum sativum L.

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00036877
AuthorSearch for: ; Search for: 1
Affiliation
  1. National Research Council of Canada. Prairie Regional Laboratory
FormatText, Article
SubjectPisum sativum; field pea; exogenous DNA; legume nodulation
Abstract
The nodulation response of a non-nodulating field pea line (Pisum sativum L., PRL-H722) following treatment with DNA from an effectively nodulating cultivar (Trapper) has been investigated. DNA was introduced to the non-nodulating cultivar via imbibition and germination of seeds in DNA solutions under aseptic conditions. The DNA preparations used were >5×10⁶ Daltons, had a smooth melting profile showing ≥40% hyperchromicity with a Tm=70–72°C in 0.1×SSC⁰ buffer, and 260/280 nm ratio of >1.8. A significant correction (ϰ²(I d.f.) = 2032**) of the mutant phenotype was observed in seeds treated with wild-type DNA. The nodulation response in treated plants was not observed in subsequent generations suggesting that the change observed did not involve integration of genetic information into the genome of germ line cells.
Publication date
PublisherSpringer Nature
In
  • Euphytica 32, no. 1 (March 1983): 171–176.
LanguageEnglish
Peer reviewedYes
IdentifierBF00036877
NRCC-20986
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Record identifier81b09c34-a067-42a5-ba0c-f6c2d227373c
Record created2023-12-04
Record modified2023-12-04
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