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The effect of exogenous DNA on the nodulation of a non-nodulating line of Pisum sativum L.

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/BF00036877
AuteurRechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Laboratoire régional des prairies
FormatTexte, Article
SujetPisum sativum; field pea; exogenous DNA; legume nodulation
Résumé
The nodulation response of a non-nodulating field pea line (Pisum sativum L., PRL-H722) following treatment with DNA from an effectively nodulating cultivar (Trapper) has been investigated. DNA was introduced to the non-nodulating cultivar via imbibition and germination of seeds in DNA solutions under aseptic conditions. The DNA preparations used were >5×10⁶ Daltons, had a smooth melting profile showing ≥40% hyperchromicity with a Tm=70–72°C in 0.1×SSC⁰ buffer, and 260/280 nm ratio of >1.8. A significant correction (ϰ²(I d.f.) = 2032**) of the mutant phenotype was observed in seeds treated with wild-type DNA. The nodulation response in treated plants was not observed in subsequent generations suggesting that the change observed did not involve integration of genetic information into the genome of germ line cells.
Date de publication
Maison d’éditionSpringer Nature
Dans
  • Euphytica 32, nº 1 (mars 1983) : 171–176.
Langueanglais
Publications évaluées par des pairsOui
IdentificateurBF00036877
NRCC-20986
Exporter la noticeExporter en format RIS
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Identificateur de l’enregistrement81b09c34-a067-42a5-ba0c-f6c2d227373c
Enregistrement créé2023-12-04
Enregistrement modifié2023-12-04
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