| Download | - View accepted manuscript: A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions (PDF, 927 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1117/12.840500 |
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| Author | Search for: Smith, Michael S. D.1; Search for: Ko, Alex C. T.1; Search for: Ridsdale, Andrew2; Search for: Schattka, Bernie1; Search for: Pegoraro, Adrian2; Search for: Hewko, Mark D.1; Search for: Shiomi, Masashi; Search for: Stolow, Albert2; Search for: Sowa, Michael G.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biodiagnostics
- National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
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| Format | Text, Article |
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| Conference | Photonics North 2009, 24 May 2009, Quebec, Canada |
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| Subject | multi-photon microscopy, atherosclerosis, two photon excited fluorescence, second harmonic generation, intrinsic fluorescence |
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| Abstract | In this study we compare the single-photon autofluorescence and multi-photon emission spectra obtained from the luminal surface of healthy segments of artery with segments where there are early atherosclerotic lesions. Arterial tissue was harvested from atherosclerosis-prone WHHL-MI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction), an animal model which mimics spontaneous myocardial infarction in humans. Single photon fluorescence emission spectra of samples were acquired using a simple spectrofluorometer set-up with 400 nm excitation. Samples were also investigated using a home built multi-photon microscope based on a Ti:sapphire femto-second oscillator. The excitation wavelength was set at 800 nm with a ~100 femto-second pulse width. Epi-multi-photon spectroscopic signals were collected through a fibre-optics coupled spectrometer. While the single-photon fluorescence spectra of atherosclerotic lesions show minimal spectroscopic difference from those of healthy arterial tissue, the multi-photon spectra collected from atherosclerotic lesions show marked changes in the relative intensity of two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) signals when compared with those from healthy arterial tissue. The observed sharp increase of the relative SHG signal intensity in a plaque is in agreement with the known pathology of early lesions which have increased collagen content. |
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| Publication date | 2009-08 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 19697995 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 8814f441-1bb0-42cf-a252-3aee48a3151d |
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| Record created | 2012-03-22 |
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| Record modified | 2020-04-16 |
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