| Téléchargement | - Voir le manuscrit accepté : A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions (PDF, 927 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1117/12.840500 |
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| Auteur | Rechercher : Smith, Michael S. D.1; Rechercher : Ko, Alex C. T.1; Rechercher : Ridsdale, Andrew2; Rechercher : Schattka, Bernie1; Rechercher : Pegoraro, Adrian2; Rechercher : Hewko, Mark D.1; Rechercher : Shiomi, Masashi; Rechercher : Stolow, Albert2; Rechercher : Sowa, Michael G.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut du biodiagnostic du CNRC
- Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
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| Format | Texte, Article |
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| Conférence | Photonics North 2009, 24 May 2009, Quebec, Canada |
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| Sujet | multi-photon microscopy, atherosclerosis, two photon excited fluorescence, second harmonic generation, intrinsic fluorescence |
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| Résumé | In this study we compare the single-photon autofluorescence and multi-photon emission spectra obtained from the luminal surface of healthy segments of artery with segments where there are early atherosclerotic lesions. Arterial tissue was harvested from atherosclerosis-prone WHHL-MI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction), an animal model which mimics spontaneous myocardial infarction in humans. Single photon fluorescence emission spectra of samples were acquired using a simple spectrofluorometer set-up with 400 nm excitation. Samples were also investigated using a home built multi-photon microscope based on a Ti:sapphire femto-second oscillator. The excitation wavelength was set at 800 nm with a ~100 femto-second pulse width. Epi-multi-photon spectroscopic signals were collected through a fibre-optics coupled spectrometer. While the single-photon fluorescence spectra of atherosclerotic lesions show minimal spectroscopic difference from those of healthy arterial tissue, the multi-photon spectra collected from atherosclerotic lesions show marked changes in the relative intensity of two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) signals when compared with those from healthy arterial tissue. The observed sharp increase of the relative SHG signal intensity in a plaque is in agreement with the known pathology of early lesions which have increased collagen content. |
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| Date de publication | 2009-08 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 19697995 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 8814f441-1bb0-42cf-a252-3aee48a3151d |
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| Enregistrement créé | 2012-03-22 |
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| Enregistrement modifié | 2020-04-16 |
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