National Research Council of Canada. Measurement Science and Standards
30th Montreaux LC/MS Symposium, 3–7 November 2014, Charleston, USA
We investigated the use of Laser Ablation Electrospray Ionization (LAESI) with MS/MS detection for Domoic Acid (DA) analysis directly from mussel tissue homogenates without sample extraction, cleanup or chromatographic separation. DA could be selectively detected directly from mussel tissue homogenates using LAESI-MS/MS in selected reaction monitoring scan mode. A matrix matched calibration curve was used to quantify mussel tissue reference materials and results were in good agreement with two established methods, LC-UV and LC-MS/MS, within the linear range. Most notable is the decrease in run time from about 20 minutes for a typical chromatographic method to about 10 seconds per sample for LAESI-MS/MS.