Germination of encapsulated embryos of interior spruce (Picea glauca engelmannii complex) and black spruce (Picea mariana Mill.)

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1007/BF00234697
Author	Search for: Lulsdorf, Monika M.; Search for: Tautorus, Thomas E.; Search for: Kikcio, Susan I.; Search for: Bethune, Terry D.; Search for: Dunstan, David I.
Format	Text, Article
Subject	sodium; sucrose; charcoal; somatic embryo; sodium alginate
Abstract	Interior spruce (Picea glauca engelmannii complex) and black spruce (Picea mariana Mill.) cotyledonary somatic embryos were encapsulated in sodium alginate. Somatic embryo viability was retained, but germination occurred at a reduced frequency compared with the equivalent zygotic embryos. The addition of 0.5% (w/v) activated charcoal to the alginate capsule significantly enhanced root development and germination for somatic embryos but not for zygotic embryos. The possibility of developing an artiflcal endosperm was also investigated, by addition of Litvay (Litvay et al. 1981) nutrients with or without 90 mM sucrose to the alginate-charcoal capsule. This treatment significantly enhanced root development for all embryo categories with the exception of black spruce somatic embryos. Encapsulated and non-encapsulated somatic embryos survived one month cold storage at 4 °C without reduction in germination frequency.
Publication date	1993-05
Publisher	Springer Nature
In	Plant Cell Reports 12-12, no. 7-8 (May 1993).
Language	English
Peer reviewed	Yes
Identifier	BF00234697 NRCC-35895
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Record identifier	96ec49af-198c-4969-97e6-72efbffae5d8
Record created	2023-11-06
Record modified	2023-11-06

Date modified:: 2024-07-20