Germination of encapsulated embryos of interior spruce (Picea glauca engelmannii complex) and black spruce (Picea mariana Mill.)

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1007/BF00234697
Auteur	Rechercher : Lulsdorf, Monika M.; Rechercher : Tautorus, Thomas E.; Rechercher : Kikcio, Susan I.; Rechercher : Bethune, Terry D.; Rechercher : Dunstan, David I.
Format	Texte, Article
Sujet	sodium; sucrose; charcoal; somatic embryo; sodium alginate
Résumé	Interior spruce (Picea glauca engelmannii complex) and black spruce (Picea mariana Mill.) cotyledonary somatic embryos were encapsulated in sodium alginate. Somatic embryo viability was retained, but germination occurred at a reduced frequency compared with the equivalent zygotic embryos. The addition of 0.5% (w/v) activated charcoal to the alginate capsule significantly enhanced root development and germination for somatic embryos but not for zygotic embryos. The possibility of developing an artiflcal endosperm was also investigated, by addition of Litvay (Litvay et al. 1981) nutrients with or without 90 mM sucrose to the alginate-charcoal capsule. This treatment significantly enhanced root development for all embryo categories with the exception of black spruce somatic embryos. Encapsulated and non-encapsulated somatic embryos survived one month cold storage at 4 °C without reduction in germination frequency.
Date de publication	1993-05
Maison d’édition	Springer Nature
Dans	Plant Cell Reports 12-12, nº 7-8 (mai 1993).
Langue	anglais
Publications évaluées par des pairs	Oui
Identificateur	BF00234697 NRCC-35895
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	96ec49af-198c-4969-97e6-72efbffae5d8
Enregistrement créé	2023-11-06
Enregistrement modifié	2023-11-06

Date de modification :: 2024-07-01