| DOI | Resolve DOI: https://doi.org/10.3390/ma6031011 |
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| Author | Search for: Zheng, D.; Search for: Vashist, S.K.; Search for: Dykas, M.M.; Search for: Saha, S.; Search for: Al-Rubeaan, K.; Search for: Lam, E.1; Search for: Luong, J.H.T.1; Search for: Sheu, F.-S. |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | 3-aminopropyltriethoxysilane; Direct electron transfer; Electrochemical glucose sensors; Glassy carbon electrodes; Glucose oxidases (GOx); Interfering substances; Pathological level; Pathophysiological; Glass membrane electrodes; Glucose; Glucose oxidase; Glucose sensors; Metabolites; Multiwalled carbon nanotubes (MWCN); Graphene |
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| Abstract | A simple procedure was developed for the fabrication of electrochemical glucose biosensors using glucose oxidase (GOx), with graphene or multi-walled carbon nanotubes (MWCNTs). Graphene and MWCNTs were dispersed in 0.25% 3-aminopropyltriethoxysilane (APTES) and drop cast on 1% KOH-pre-treated glassy carbon electrodes (GCEs). The EDC (1-ethyl-(3-dimethylaminopropyl) carbodiimide)-activated GOx was then bound covalently on the graphene- or MWCNT-modified GCE. Both the graphene- and MWCNT-based biosensors detected the entire pathophysiological range of blood glucose in humans, 1.4-27.9 mM. However, the direct electron transfer (DET) between GOx and the modified GCE's surface was only observed for the MWCNT-based biosensor. The MWCNT-based glucose biosensor also provided over a four-fold higher current signal than its graphene counterpart. Several interfering substances, including drug metabolites, provoked negligible interference at pathological levels for both the MWCNT- and graphene-based biosensors. However, the former was more prone to interfering substances and drug metabolites at extremely pathological concentrations than its graphene counterpart. © 2013 by the authors. |
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| Publication date | 2013 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270620 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 99416130-3392-4976-8d08-922d691f53a7 |
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| Record created | 2014-02-17 |
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| Record modified | 2020-04-22 |
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