| DOI | Trouver le DOI : https://doi.org/10.3390/ma6031011 |
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| Auteur | Rechercher : Zheng, D.; Rechercher : Vashist, S.K.; Rechercher : Dykas, M.M.; Rechercher : Saha, S.; Rechercher : Al-Rubeaan, K.; Rechercher : Lam, E.1; Rechercher : Luong, J.H.T.1; Rechercher : Sheu, F.-S. |
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| Affiliation | - Conseil national de recherches du Canada
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| Format | Texte, Article |
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| Sujet | 3-aminopropyltriethoxysilane; Direct electron transfer; Electrochemical glucose sensors; Glassy carbon electrodes; Glucose oxidases (GOx); Interfering substances; Pathological level; Pathophysiological; Glass membrane electrodes; Glucose; Glucose oxidase; Glucose sensors; Metabolites; Multiwalled carbon nanotubes (MWCN); Graphene |
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| Résumé | A simple procedure was developed for the fabrication of electrochemical glucose biosensors using glucose oxidase (GOx), with graphene or multi-walled carbon nanotubes (MWCNTs). Graphene and MWCNTs were dispersed in 0.25% 3-aminopropyltriethoxysilane (APTES) and drop cast on 1% KOH-pre-treated glassy carbon electrodes (GCEs). The EDC (1-ethyl-(3-dimethylaminopropyl) carbodiimide)-activated GOx was then bound covalently on the graphene- or MWCNT-modified GCE. Both the graphene- and MWCNT-based biosensors detected the entire pathophysiological range of blood glucose in humans, 1.4-27.9 mM. However, the direct electron transfer (DET) between GOx and the modified GCE's surface was only observed for the MWCNT-based biosensor. The MWCNT-based glucose biosensor also provided over a four-fold higher current signal than its graphene counterpart. Several interfering substances, including drug metabolites, provoked negligible interference at pathological levels for both the MWCNT- and graphene-based biosensors. However, the former was more prone to interfering substances and drug metabolites at extremely pathological concentrations than its graphene counterpart. © 2013 by the authors. |
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| Date de publication | 2013 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21270620 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 99416130-3392-4976-8d08-922d691f53a7 |
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| Enregistrement créé | 2014-02-17 |
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| Enregistrement modifié | 2020-04-22 |
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