| DOI | Resolve DOI: https://doi.org/10.1006/bbrc.1997.7908 |
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| Author | Search for: Gruwel, Marco L.H.1; Search for: Čulić, Ognjen; Search for: Muhs, Andreas; Search for: Williams, John P.; Search for: Schrader, Jürgen |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Abstract | Using anin vitrocell system and Cs⁺NMR techniques we were able to show that porcine aortic endothelial cells (PAEC) reduce their Na⁺-K⁺-ATPase activity upon an increase in intracellular cAMP. Reduction in the pump rate was due to phosphorylation of the α-subunit of the ATPase as shown by immunoprecipitation. Apart from a pump inhibiton using 8-Br-cAMP and IBMX, we were also able to show that changes in the Na⁺-K⁺-ATPase activity could be mediated by the adenosine-A₂and prostaglandin receptor agonists 5′-N-Ethylcarboxamidoadenosine and Iloprost, respectively. Parallel to a decrease in pump activity we also observed a decrease in intracellular Cs⁺, indicating opening of K⁺channels. |
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| Publication date | 1998-01 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | S0006291X97979080 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | b3783b77-f367-49d6-be99-7a27fd5be945 |
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| Record created | 2023-11-01 |
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| Record modified | 2023-11-01 |
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