Sucrose requirements and lipid utilization during germination of interior spruce (Picea glauca engelmannii complex) somatic embryos

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1007/BF01142322
Author	Search for: Carrier, D. J.¹; Search for: Cunningham, J. E.¹; Search for: Taylor, D. C.¹; Search for: Dunstan, D. I.¹
Affiliation	National Research Council of Canada. NRC Plant Biotechnology Institute
Format	Text, Article
Subject	carbohydrates; fatty acid; germination; somatic embryogenesis
Abstract	Both somatic and excised zygotic embryos of interior spruce (Picea glauca engelmannii complex) required exogenous sucrose in the medium for germination in vitro. Over a period of 29 days on sucrose-containing medium germinants with roots and epicotyls developed from both kinds of embryo, and their content of linolenic acid (9,12,15-18:3) increased about six- to eightfold. Without added sucrose, embryos showed retarded growth or were necrotic, and the content of linolenic acid was barely detectable in their fatty acid profiles. Through ¹⁴C-sucrose uptake studies, it was determined that germinants consumed only 25% of the sucrose available in a 1% (wt/vol) sucrose-containing medium. Since no radiolabelled fatty acids were detected, it appears that externally supplied sucrose was not used in the synthesis of lipids. Although sucrose was present during plantlet development, 72% of the initial lipids were consumed. To some extent, the plantlets appeared to be obligate storage lipid utilizers.
Publication date	1997-05
Publisher	Springer Link
In	Plant Cell Reports 16, no. 8 (May 1997): 550–554.
Language	English
Peer reviewed	Yes
Identifier	BF01142322 NRCC-40028
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Record identifier	b3a97feb-7ce3-4456-a779-3dfdab9331b3
Record created	2023-11-01
Record modified	2023-11-01

Date modified:: 2025-06-23