Sucrose requirements and lipid utilization during germination of interior spruce (Picea glauca engelmannii complex) somatic embryos

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1007/BF01142322
Auteur	Rechercher : Carrier, D. J.¹; Rechercher : Cunningham, J. E.¹; Rechercher : Taylor, D. C.¹; Rechercher : Dunstan, D. I.¹
Affiliation	Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
Format	Texte, Article
Sujet	carbohydrates; fatty acid; germination; somatic embryogenesis
Résumé	Both somatic and excised zygotic embryos of interior spruce (Picea glauca engelmannii complex) required exogenous sucrose in the medium for germination in vitro. Over a period of 29 days on sucrose-containing medium germinants with roots and epicotyls developed from both kinds of embryo, and their content of linolenic acid (9,12,15-18:3) increased about six- to eightfold. Without added sucrose, embryos showed retarded growth or were necrotic, and the content of linolenic acid was barely detectable in their fatty acid profiles. Through ¹⁴C-sucrose uptake studies, it was determined that germinants consumed only 25% of the sucrose available in a 1% (wt/vol) sucrose-containing medium. Since no radiolabelled fatty acids were detected, it appears that externally supplied sucrose was not used in the synthesis of lipids. Although sucrose was present during plantlet development, 72% of the initial lipids were consumed. To some extent, the plantlets appeared to be obligate storage lipid utilizers.
Date de publication	1997-05
Maison d’édition	Springer Link
Dans	Plant Cell Reports 16, nº 8 (mai 1997) : 550–554.
Langue	anglais
Publications évaluées par des pairs	Oui
Identificateur	BF01142322 NRCC-40028
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	b3a97feb-7ce3-4456-a779-3dfdab9331b3
Enregistrement créé	2023-11-01
Enregistrement modifié	2023-11-01

Date de modification :: 2024-07-03