Sélection de la langue

Gouvernement du Canada / Government of Canada

Recherche

Sucrose requirements and lipid utilization during germination of interior spruce (Picea glauca engelmannii complex) somatic embryos

DOITrouver le DOI : https://doi.org/10.1007/BF01142322
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
Sujetcarbohydrates; fatty acid; germination; somatic embryogenesis
Résumé
Both somatic and excised zygotic embryos of interior spruce (Picea glauca engelmannii complex) required exogenous sucrose in the medium for germination in vitro. Over a period of 29 days on sucrose-containing medium germinants with roots and epicotyls developed from both kinds of embryo, and their content of linolenic acid (9,12,15-18:3) increased about six- to eightfold. Without added sucrose, embryos showed retarded growth or were necrotic, and the content of linolenic acid was barely detectable in their fatty acid profiles. Through ¹⁴C-sucrose uptake studies, it was determined that germinants consumed only 25% of the sucrose available in a 1% (wt/vol) sucrose-containing medium. Since no radiolabelled fatty acids were detected, it appears that externally supplied sucrose was not used in the synthesis of lipids. Although sucrose was present during plantlet development, 72% of the initial lipids were consumed. To some extent, the plantlets appeared to be obligate storage lipid utilizers.
Date de publication
Maison d’éditionSpringer Link
Dans
  • Plant Cell Reports 16, nº 8 (mai 1997) : 550–554.
Langueanglais
Publications évaluées par des pairsOui
IdentificateurBF01142322
NRCC-40028
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrementb3a97feb-7ce3-4456-a779-3dfdab9331b3
Enregistrement créé2023-11-01
Enregistrement modifié2023-11-01

Détails de la page

Par :

Date de modification :