Identification of pectenotoxin-11 as 34S-hydroxypectenotoxin-2, a new pectenotoxin analogue in the toxic donoflagellate Dinophysis acuta from New Zealand

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1021/tx050240y
Author	Search for: Suzuki, Toshiyuki¹; Search for: Walter, John A.²; Search for: LeBlanc, Patricia²; Search for: MacKinnon, Shawna³; Search for: Miles, Christopher O.; Search for: Wilkins, Alistair L.; Search for: Munday, Rex; Search for: Beuzenberg, Veronica; Search for: MacKenzie, A. Lincoln; Search for: Jensen, Dwayne J.; Search for: Cooney, Janine M.; Search for: Quilliam, Michael A.²
Affiliation	National Research Council of Canada National Research Council of Canada. Measurement Science and Standards National Research Council of Canada. Aquatic and Crop Resource Development
Format	Text, Article
Subject	biological poisons; coasts; diarrhea; dinoflagellates; enzymes; hepatopancreas; magnetic resonance spectroscopy; marine molluscs; mass spectroscopy; mortality causes; phytoplankton; public health; toxicants; toxicity; toxicity tests; ultraviolet radiation; pectenotoxins; Perna canaliculus; Dinophysis acuta
Abstract	A new pectenotoxin, which has been named pectenotoxin-11 (PTX11), was isolated from the dinoflagellate Dinophysis acuta collected from the west coast of New Zealand. The structure of PTX11 was determined as 34S-hydroxypectenotoxin-2 by tandem mass spectrometry and UV and NMR spectroscopy. PTX11 appears to be only the third pectenotoxin identified as a natural biosynthetic product from algae after pectenotoxin-2 and pectenotoxin-12. The LD50 of PTX11 determined by mouse intraperitoneal injection was 244 g/kg. The LDmin of PTX11 in these experiments was 250 g/kg. No signs of toxicity were recorded in mice following an oral dose of PTX11 at 5000 g/kg. No diarrhea was observed in any of the animals administered with the test substance by either route of administration. Unlike pectenotoxin-2 (PTX2), PTX11 was not readily hydrolyzed to its corresponding seco acid by enzymes from homogenized green-lipped mussel (Perna canaliculus) hepatopancreas.
Publication date	2007-08-01
Terms of use	Copyright © 2006 American Chemical Society
In	Chemical Research in Toxicology 19, no. 2 (1 August 2007): 310–318.
Language	English
Peer reviewed	Yes
NRC number	NRCC 42555 1628
NPARC number	3538365
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Record identifier	b4b1270b-03a4-4c00-8dc6-86c38a7e9d52
Record created	2009-03-01
Record modified	2020-05-10

Date modified:: 2024-12-21