Identification of pectenotoxin-11 as 34S-hydroxypectenotoxin-2, a new pectenotoxin analogue in the toxic donoflagellate Dinophysis acuta from New Zealand

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1021/tx050240y
Auteur	Rechercher : Suzuki, Toshiyuki¹; Rechercher : Walter, John A.²; Rechercher : LeBlanc, Patricia²; Rechercher : MacKinnon, Shawna³; Rechercher : Miles, Christopher O.; Rechercher : Wilkins, Alistair L.; Rechercher : Munday, Rex; Rechercher : Beuzenberg, Veronica; Rechercher : MacKenzie, A. Lincoln; Rechercher : Jensen, Dwayne J.; Rechercher : Cooney, Janine M.; Rechercher : Quilliam, Michael A.²
Affiliation	Conseil national de recherches du Canada Conseil national de recherches du Canada. Science des mesures et étalons Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
Format	Texte, Article
Sujet	biological poisons; coasts; diarrhea; dinoflagellates; enzymes; hepatopancreas; magnetic resonance spectroscopy; marine molluscs; mass spectroscopy; mortality causes; phytoplankton; public health; toxicants; toxicity; toxicity tests; ultraviolet radiation; pectenotoxins; Perna canaliculus; Dinophysis acuta
Résumé	A new pectenotoxin, which has been named pectenotoxin-11 (PTX11), was isolated from the dinoflagellate Dinophysis acuta collected from the west coast of New Zealand. The structure of PTX11 was determined as 34S-hydroxypectenotoxin-2 by tandem mass spectrometry and UV and NMR spectroscopy. PTX11 appears to be only the third pectenotoxin identified as a natural biosynthetic product from algae after pectenotoxin-2 and pectenotoxin-12. The LD50 of PTX11 determined by mouse intraperitoneal injection was 244 g/kg. The LDmin of PTX11 in these experiments was 250 g/kg. No signs of toxicity were recorded in mice following an oral dose of PTX11 at 5000 g/kg. No diarrhea was observed in any of the animals administered with the test substance by either route of administration. Unlike pectenotoxin-2 (PTX2), PTX11 was not readily hydrolyzed to its corresponding seco acid by enzymes from homogenized green-lipped mussel (Perna canaliculus) hepatopancreas.
Date de publication	2007-08-01
Conditions d’utilisation	Copyright © 2006 American Chemical Society
Dans	Chemical Research in Toxicology 19, nº 2 (1 août 2007) : 310–318.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	NRCC 42555 1628
Numéro NPARC	3538365
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	b4b1270b-03a4-4c00-8dc6-86c38a7e9d52
Enregistrement créé	2009-03-01
Enregistrement modifié	2020-05-10

Date de modification :: 2024-12-21