| DOI | Resolve DOI: https://doi.org/10.1021/ac048385h |
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| Author | Search for: Burton, Ian W.1; Search for: Quilliam, Michael A.2; Search for: Walter, John A.2 |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
- National Research Council of Canada. Measurement Science and Standards
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| Format | Text, Article |
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| Subject | hazardous materials; metabolites; N.M.R.; probes; solvents; toxicants; toxins; natural products |
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| Abstract | We examine the use of external standards for quantitative measurement by 1H NMR of solution concentrations of natural products and other low molecular weight, hydrogen-containing compounds and show that precision and accuracy ca. 1% is obtainable with a commercial 11.7 T spectrometer when standards and analytes are contained in separate but identical sealed precision glass NMR tubes. Numerous factors contributing to the intensity of the NMR signals are evaluated. Precise measurements of 360 pulse lengths for each sample provide direct corrections for variations in probe Q-factor that enable samples in different solvents to be compared, provided single-coil excitation and detection is used throughout. Samples need not be prepared in deuterated solvents if the 1H spectra of the solvents are simple enough for peak suppression by presaturation. The approach is particularly suitable for hazardous materials kept in sealed tubes and for the preparation of certified calibration solution reference materials for use with LC-MS and other techniques where deuterated solvents should be avoided. |
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| Publication date | 2005 |
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| Terms of use | - Copyright © 2007 American Chemical Society
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | 1324 |
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| NPARC number | 3538057 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | b509bae8-2762-458c-8e0b-ac4d55d15b6a |
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| Record created | 2009-03-01 |
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| Record modified | 2020-04-07 |
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