| DOI | Trouver le DOI : https://doi.org/10.1021/ac048385h |
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| Auteur | Rechercher : Burton, Ian W.1; Rechercher : Quilliam, Michael A.2; Rechercher : Walter, John A.2 |
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| Affiliation du nom | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
- Conseil national de recherches du Canada. Science des mesures et étalons
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| Format | Texte, Article |
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| Sujet | hazardous materials; metabolites; N.M.R.; probes; solvents; toxicants; toxins; natural products |
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| Résumé | We examine the use of external standards for quantitative measurement by 1H NMR of solution concentrations of natural products and other low molecular weight, hydrogen-containing compounds and show that precision and accuracy ca. 1% is obtainable with a commercial 11.7 T spectrometer when standards and analytes are contained in separate but identical sealed precision glass NMR tubes. Numerous factors contributing to the intensity of the NMR signals are evaluated. Precise measurements of 360 pulse lengths for each sample provide direct corrections for variations in probe Q-factor that enable samples in different solvents to be compared, provided single-coil excitation and detection is used throughout. Samples need not be prepared in deuterated solvents if the 1H spectra of the solvents are simple enough for peak suppression by presaturation. The approach is particularly suitable for hazardous materials kept in sealed tubes and for the preparation of certified calibration solution reference materials for use with LC-MS and other techniques where deuterated solvents should be avoided. |
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| Date de publication | 2005 |
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| Conditions d’utilisation | - Copyright © 2007 American Chemical Society
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | 1324 |
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| Numéro NPARC | 3538057 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | b509bae8-2762-458c-8e0b-ac4d55d15b6a |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-04-07 |
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