| DOI | Resolve DOI: https://doi.org/10.1007/978-1-61779-968-6-27 |
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| Author | Search for: Zhang, J.1; Search for: MacKenzie, C.R.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | carcinoembryonic antigen related cell adhesion molecule 1; nanobody; pentabody ES1; plasmid vector; unclassified drug; nanobody; antigen binding; antigen recognition; carcinoma cell; drug purification; drug synthesis; gene expression; human cell; nucleotide sequence; phage display; amino acid sequence; Artiodactyla; cell surface display; chemistry; gene expression; gene library; gene order; genetics; immunoglobulin variable region; immunology; methodology; molecular genetics; protein binding; tumor cell line; Amino Acid Sequence; Camelids, New World; Cell Line, Tumor; Cell Surface Display Techniques; Gene Expression; Gene Library; Gene Order; Immunoglobulin Variable Region; Molecular Sequence Data; Protein Binding; Single-Domain Antibodies |
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| Abstract | Antigen-binding fragments, such as single-domain antibodies (sdAbs), can now be readily isolated by in vitro technologies. Antibody fragment libraries derived from immune or nonimmune sources are presented in a molecular display format, typically phage display, and binders to individual antigens are selected from the libraries by a so-called panning process. Nonimmune libraries can serve as sources of binders to a wide range of targets but yield antigen-binding fragments that generally have much lower affinities than those obtained from immune sources. Here we describe a strategy for constructing pentameric sdAbs termed pentabodies. Pentamerization introduces avidity which can greatly enhance the binding of low affinity sdAbs to antigens presented on surfaces. © 2012 Springer Science+Business Media, LLC. |
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| Publication date | 2012 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21269170 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | b82ea504-ebdc-41a0-82c8-969f6e608e89 |
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| Record created | 2013-12-12 |
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| Record modified | 2020-04-21 |
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