| DOI | Trouver le DOI : https://doi.org/10.1007/978-1-61779-968-6-27 |
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| Auteur | Rechercher : Zhang, J.1; Rechercher : MacKenzie, C.R.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | carcinoembryonic antigen related cell adhesion molecule 1; nanobody; pentabody ES1; plasmid vector; unclassified drug; nanobody; antigen binding; antigen recognition; carcinoma cell; drug purification; drug synthesis; gene expression; human cell; nucleotide sequence; phage display; amino acid sequence; Artiodactyla; cell surface display; chemistry; gene expression; gene library; gene order; genetics; immunoglobulin variable region; immunology; methodology; molecular genetics; protein binding; tumor cell line; Amino Acid Sequence; Camelids, New World; Cell Line, Tumor; Cell Surface Display Techniques; Gene Expression; Gene Library; Gene Order; Immunoglobulin Variable Region; Molecular Sequence Data; Protein Binding; Single-Domain Antibodies |
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| Résumé | Antigen-binding fragments, such as single-domain antibodies (sdAbs), can now be readily isolated by in vitro technologies. Antibody fragment libraries derived from immune or nonimmune sources are presented in a molecular display format, typically phage display, and binders to individual antigens are selected from the libraries by a so-called panning process. Nonimmune libraries can serve as sources of binders to a wide range of targets but yield antigen-binding fragments that generally have much lower affinities than those obtained from immune sources. Here we describe a strategy for constructing pentameric sdAbs termed pentabodies. Pentamerization introduces avidity which can greatly enhance the binding of low affinity sdAbs to antigens presented on surfaces. © 2012 Springer Science+Business Media, LLC. |
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| Date de publication | 2012 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21269170 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | b82ea504-ebdc-41a0-82c8-969f6e608e89 |
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| Enregistrement créé | 2013-12-12 |
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| Enregistrement modifié | 2020-04-21 |
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