| Download | - View accepted manuscript: Molecular characterization of microbial communities in Canadian pulp and paper activated sludge and quantification of a novel Thiothrix eikelboomii -like bulking filament (PDF, 1.2 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1139/w05-160 |
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| Author | Search for: Dumonceaux, Tim J; Search for: Hill, Janet E1; Search for: Pelletier, Carl P; Search for: Paice, Michael G; Search for: Van Kessel, Andrew G; Search for: Hemmingsen, Sean M1 |
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| Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
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| Format | Text, Article |
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| Subject | activated sludge; biological treatment; bulking; chaperonin 60; cpn60; filamentous bacteria; mixed liquor suspended solids; microbial communities; 021N; qPCR; settling; Thiothrix |
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| Abstract | We examined the microbial community structure and quantified the levels of the filamentous bulking organism Thiothrix eikelboomii in samples of activated sludge mixed liquor suspended solids (MLSS) from Canadian pulp and paper mills. Libraries of chaperonin 60 (cpn60) gene sequences were prepared from MLSS total microbial community DNA and each was compared with cpnDB, a reference database of cpn60 sequences (http://cpndb.cbr.nrc.ca) for assignment of taxonomic identities. Sequences similar to but distinct from the type strain of T. eikelboomii AP3 (ATCC 49788ᵀ) (~89% identity over 555 bp) were recovered at high frequency from a mill sample that was experiencing bulking problems at the time of sample collection, which corresponded to microscopic observations using fluorescent in situ hybridization with commercially available 16S rDNA-based probes. We enumerated this strain in five mill-derived MLSS samples using real-time quantitative PCR (qPCR) and found that two samples had high levels of the bulking strain (>10¹² genomes/g MLSS) and two contained lower but detectable levels of this organism. None of the mill samples contained cpn60 sequences that were identical to the type strain of T. eikelboomii. This technique shows promise for monitoring pulp and paper mill wastewater treatment systems by detecting and enumerating this strain of T. eikelboomii, which may be specific to pulp and paper mill wastewater treatment systems. |
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| Publication date | 2006-05-01 |
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| Publisher | Canadian Science Publishing |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | be1152d3-f534-462e-bc75-bfee8df80266 |
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| Record created | 2023-10-18 |
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| Record modified | 2024-05-22 |
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