Determination of underivatized amino acids in microsamples of a yeast nutritional supplement by LC-MS following microwave assisted acid hydrolysis

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1039/C6AY00407E
AuthorSearch for: 1; Search for: 1; Search for: 1ORCID identifier: https://orcid.org/0000-0002-2377-2615
Name affiliation
  1. National Research Council Canada. Measurement Science and Standards
FormatText
TypeArticle
Journal titleAnalytical Methods
ISSN1759-9660
1759-9679
Volume8
Issue22
Pages4497–4503
Abstract
In this paper we describe a rapid method for microscale microwave assisted acid hydrolysis followed by quantitative amino acid analysis, using liquid chromatography mass spectrometry UPLC-ESI-MS (QTOF) without derivatization. Such an approach allows substantial decrease in the amount of yeast sample (less than 1 mg) required for effective analysis. The vapor phase hydrolysis approach was compared to condensed phase hydrolysis and all the microwave parameters were optimized for 16 amino acids. Separation was achieved on a BEH C-18 column. Limits of detection and quantitation (LODs/LOQs), using standard solutions, were in the sub-ppb range. The method was successfully applied to the determination and quantitation of methionine and selenomethionine by isotope dilution and standard addition methods in yeast on a yeast certified reference material sample.
Publication date
PublisherRoyal Society of Chemistry
LanguageEnglish
Peer reviewedYes
NPARC number23000365
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