| Alternative title | Abstracts presented at the 37th Annual Meeting of The Protein Society. ABS#653: Site-specific incorporation of 3-methyl-histidine into Tannase |
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| Author | Search for: Chevannes, Deandre; Search for: St-Jacques, Antony1; Search for: Das, Prem1ORCID identifier: https://orcid.org/0000-0001-8044-0425; Search for: Lister, Joshua; Search for: Rajagopalan, Nandhakishore1; Search for: Loewen, Matthew Loewen1; Search for: Loewen, Michele1 |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Abstract |
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| Conference | 37th Annual Meeting of The Protein Society, July 13-16, 2023, Boston, MA, USA |
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| Abstract | Tannin acyl hydrolase or tannase functions as an esterase that catalyzes the hydrolysis of ester bonds in gallo tannins producing gallic acid and its derivates. Tannase is used in a variety of different industries such as food, chemical, agriculture, and pharmaceutical and thus represents an important target for biocatalytic development. Recent studies have emphasized the potential of genetic code expansion (GCE) to modify proteins and increase catalytic activity of enzymes. GCE allows for the incorporation of non-canonical amino acids. In this study the impact of replacing the H451 in the esterase catalytic triad with its non-canonical counterpart 3-methyl-histidine(3mH) was evaluated. A decreased reliance on the Asp of the catalytic triad, while shortening the distance between the His and the Ser was proposed to create a more efficient catalytic dyad. A pyrrolysltRNA synthetase (PyIRS)/ tRNAPyl pair were used in combination with a tannase mutant encoding an amber stop codon. The activity of the derived NmH451 tannase was compared to wild-type and unexpectedly showed a decrease in activity. Mass spectrophotometry is currently being applied to analyze accuracy of incorporation. |
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| Publication date | 2023-11-27 |
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| Publisher | Wiley |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | c52060c8-1296-421c-b7fb-205cd94f1a1f |
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| Record created | 2024-06-07 |
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| Record modified | 2024-06-07 |
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