| Autre titre | Abstracts presented at the 37th Annual Meeting of The Protein Society. ABS#653: Site-specific incorporation of 3-methyl-histidine into Tannase |
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| Auteur | Rechercher : Chevannes, Deandre; Rechercher : St-Jacques, Antony1; Rechercher : Das, Prem1Identifiant ORCID : https://orcid.org/0000-0001-8044-0425; Rechercher : Lister, Joshua; Rechercher : Rajagopalan, Nandhakishore1; Rechercher : Loewen, Matthew Loewen1; Rechercher : Loewen, Michele1 |
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| Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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| Format | Texte, Résumé |
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| Conférence | 37th Annual Meeting of The Protein Society, July 13-16, 2023, Boston, MA, USA |
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| Résumé | Tannin acyl hydrolase or tannase functions as an esterase that catalyzes the hydrolysis of ester bonds in gallo tannins producing gallic acid and its derivates. Tannase is used in a variety of different industries such as food, chemical, agriculture, and pharmaceutical and thus represents an important target for biocatalytic development. Recent studies have emphasized the potential of genetic code expansion (GCE) to modify proteins and increase catalytic activity of enzymes. GCE allows for the incorporation of non-canonical amino acids. In this study the impact of replacing the H451 in the esterase catalytic triad with its non-canonical counterpart 3-methyl-histidine(3mH) was evaluated. A decreased reliance on the Asp of the catalytic triad, while shortening the distance between the His and the Ser was proposed to create a more efficient catalytic dyad. A pyrrolysltRNA synthetase (PyIRS)/ tRNAPyl pair were used in combination with a tannase mutant encoding an amber stop codon. The activity of the derived NmH451 tannase was compared to wild-type and unexpectedly showed a decrease in activity. Mass spectrophotometry is currently being applied to analyze accuracy of incorporation. |
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| Date de publication | 2023-11-27 |
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| Maison d’édition | Wiley |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | c52060c8-1296-421c-b7fb-205cd94f1a1f |
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| Enregistrement créé | 2024-06-07 |
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| Enregistrement modifié | 2024-06-07 |
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