| DOI | Resolve DOI: https://doi.org/10.1016/j.carres.2017.01.009 |
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| Author | Search for: Vinogradov, Evgeny1; Search for: St. Michael, Frank1; Search for: Homma, Kiyonobu; Search for: Sharma, Ashu; Search for: Cox, Andrew D.1 |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Subject | Fusobacterium nucleatum; lipopolysaccharide; sialic acid |
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| Abstract | Fusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. Recently, it has been gaining attention as a potential causative agent for colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. Little is known about virulence factors of this organism and thus we have initiated studies to examine the bacterial surface glycochemistry. Consistent with a recent paper suggesting that F. nucleatum strain 10593 can synthesize sialic acid, a staining technique identified sialic acid on the bacterial surface. We isolated lipopolysaccharide from this F. nucleatum strain and performed structural analysis on the O-antigen. Our studies identified a trisaccharide repeating unit of the O-antigen with the following structure:
-[→4)-α-Neup5Ac-(2 → 4)-β-d-Galp-(1 → 3)-α-d-FucpNAc4NAc-(1-]-
where Ac indicates 4-N-acetylation of ∼30% FucNAc4N residues.
The presence of sialic acid as a constituent of the O-antigen is consistent with recent data identifying de novo sialic acid synthesis in this strain. |
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| Publication date | 2017-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23001481 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | c66e99af-5337-492e-8f3f-7716ef0e2abd |
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| Record created | 2017-02-17 |
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| Record modified | 2020-03-16 |
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