| DOI | Trouver le DOI : https://doi.org/10.1016/j.carres.2017.01.009 |
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| Auteur | Rechercher : Vinogradov, Evgeny1; Rechercher : St. Michael, Frank1; Rechercher : Homma, Kiyonobu; Rechercher : Sharma, Ashu; Rechercher : Cox, Andrew D.1 |
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| Affiliation | - Conseil national de recherches Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Sujet | Fusobacterium nucleatum; lipopolysaccharide; sialic acid |
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| Résumé | Fusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. Recently, it has been gaining attention as a potential causative agent for colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. Little is known about virulence factors of this organism and thus we have initiated studies to examine the bacterial surface glycochemistry. Consistent with a recent paper suggesting that F. nucleatum strain 10593 can synthesize sialic acid, a staining technique identified sialic acid on the bacterial surface. We isolated lipopolysaccharide from this F. nucleatum strain and performed structural analysis on the O-antigen. Our studies identified a trisaccharide repeating unit of the O-antigen with the following structure:
-[→4)-α-Neup5Ac-(2 → 4)-β-d-Galp-(1 → 3)-α-d-FucpNAc4NAc-(1-]-
where Ac indicates 4-N-acetylation of ∼30% FucNAc4N residues.
The presence of sialic acid as a constituent of the O-antigen is consistent with recent data identifying de novo sialic acid synthesis in this strain. |
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| Date de publication | 2017-01 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23001481 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | c66e99af-5337-492e-8f3f-7716ef0e2abd |
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| Enregistrement créé | 2017-02-17 |
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| Enregistrement modifié | 2020-03-16 |
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