| DOI | Resolve DOI: https://doi.org/10.1038/nchembio.525 |
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| Author | Search for: Pezacki, J.P.1; Search for: Blake, J.A.1; Search for: Danielson, D.C.1; Search for: Kennedy, D.C.1; Search for: Lyn, R.K.1; Search for: Singaravelu, R.1 |
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| Affiliation | - National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
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| Format | Text, Article |
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| Subject | adipogenesis; cell labeling; cell organelle; coherent anti stokes raman scattering microscopy; environment; Hepatitis C virus; host pathogen interaction; imaging; life cycle; lipid metabolism; lipid storage; microscopy; molecule; review; tissue; vibration; Cell Tracking; Contrast Media; Lipid Metabolism; Molecular Imaging; Spectrum Analysis, Raman; Vibration |
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| Abstract | Cellular biomolecules contain unique molecular vibrations that can be visualized by coherent anti-Stokes Raman scattering (CARS) microscopy without the need for labels. Here we review the application of CARS microscopy for label-free imaging of cells and tissues using the natural vibrational contrast that arises from biomolecules like lipids as well as for imaging of exogenously added probes or drugs. High-resolution CARS microscopy combined with multimodal imaging has allowed for dynamic monitoring of cellular processes such as lipid metabolism and storage, the movement of organelles, adipogenesis and host-pathogen interactions and can also be used to track molecules within cells and tissues. The CARS imaging modality provides a unique tool for biological chemists to elucidate the state of a cellular environment without perturbing it and to perceive the functional effects of added molecules. © 2011 Nature America, Inc. All rights reserved. |
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| Publication date | 2011 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21271377 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | cc4cc03c-1a9a-4b72-8dd5-8778423a888a |
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| Record created | 2014-03-24 |
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| Record modified | 2020-04-21 |
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