DOI | Trouver le DOI : https://doi.org/10.1038/nchembio.525 |
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Auteur | Rechercher : Pezacki, J.P.1; Rechercher : Blake, J.A.1; Rechercher : Danielson, D.C.1; Rechercher : Kennedy, D.C.1; Rechercher : Lyn, R.K.1; Rechercher : Singaravelu, R.1 |
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Affiliation | - Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
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Format | Texte, Article |
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Sujet | adipogenesis; cell labeling; cell organelle; coherent anti stokes raman scattering microscopy; environment; Hepatitis C virus; host pathogen interaction; imaging; life cycle; lipid metabolism; lipid storage; microscopy; molecule; review; tissue; vibration; Cell Tracking; Contrast Media; Lipid Metabolism; Molecular Imaging; Spectrum Analysis, Raman; Vibration |
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Résumé | Cellular biomolecules contain unique molecular vibrations that can be visualized by coherent anti-Stokes Raman scattering (CARS) microscopy without the need for labels. Here we review the application of CARS microscopy for label-free imaging of cells and tissues using the natural vibrational contrast that arises from biomolecules like lipids as well as for imaging of exogenously added probes or drugs. High-resolution CARS microscopy combined with multimodal imaging has allowed for dynamic monitoring of cellular processes such as lipid metabolism and storage, the movement of organelles, adipogenesis and host-pathogen interactions and can also be used to track molecules within cells and tissues. The CARS imaging modality provides a unique tool for biological chemists to elucidate the state of a cellular environment without perturbing it and to perceive the functional effects of added molecules. © 2011 Nature America, Inc. All rights reserved. |
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Date de publication | 2011 |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 21271377 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | cc4cc03c-1a9a-4b72-8dd5-8778423a888a |
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Enregistrement créé | 2014-03-24 |
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Enregistrement modifié | 2020-04-21 |
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