| DOI | Resolve DOI: https://doi.org/10.1139/m96-110 |
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| Author | Search for: Juck, David; Search for: Ingram, Jordan; Search for: Prévost, Michèle; Search for: Coallier, Josée; Search for: Greer, Charles1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | environmental; nested PCR; sensitive; detection; potable water; sensibilité; détection; eau potable |
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| Abstract | A rapid and sensitive method for the detection of low levels of bacteria in potable water was developed. The fecal indicator bacterium Escherichia coli was used as the test organism in a filtration concentration-nested polymerase chain reaction (PCR) protocol, combined with ethidium bromide visualization of PCR products. Two sets of primers were designed from the E. coli specific β-glucuronidase gene (uidA), the primary pair producing a 486-bp fragment that was used as template for the nested primer pair delineating a 186-bp fragment. This protocol can detect 1-10 bacterial cells/50 mL water sample within 6-8 h, in contrast to traditional culturing or Southern hybridization methods which require 2-3 days for results. |
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| Publication date | 1996 |
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| In | |
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| Language | English |
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| NRC number | 34557 |
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| NPARC number | 3539699 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | ce75604f-3a25-4e60-a76e-8231c13616b6 |
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| Record created | 2009-03-01 |
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| Record modified | 2020-03-20 |
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