| Download | - View final version: Is clathrin involved in internalization of the small molecule berberine by primary mouse mast cells? (PDF, 953 KiB)
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| DOI | Resolve DOI: https://doi.org/10.26502/ami.93650082 |
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| Author | Search for: Alam, Syed Benazir1; Search for: Kulka, Marianna1 |
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| Affiliation | - National Research Council of Canada. Nanotechnology
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| Format | Text, Article |
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| Subject | endocytosis; mast cells; interleukin-3; berberine; cell cycle; clathrin |
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| Abstract | The internalization and endocytosis of small molecules is a process that relies on either passive diffusion across the cell membrane or active transport through complex endocytic pathways such as pinocytosis. Using the fluorescent benzylisoquinoline compound berberine (BBR) as a tool, we analyzed the internalization of this small molecule by differentiated mouse bone marrow-derived mast cells. Our recent data showed that internalization of this compound was energy-and time-dependent and was increased when cells were treated with the differentiation cytokine, interleukin-3 (IL-3). In this commentary, we examine some of the possible mechanisms of internalization and provide further data to show that this process is partially dependent on clathrin-mediated endocytosis. Our hypothesis is that BBR internalization may utilize several pathways of internalization depending upon cell activation and proliferation. |
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| Publication date | 2022-04-26 |
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| Publisher | Fortune Journals |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-NANO-225 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | d8374487-9d84-45ca-b5f2-ea4f44bba99a |
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| Record created | 2023-01-03 |
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| Record modified | 2023-01-03 |
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