| Download | - View accepted manuscript: Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode (PDF, 1.7 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1021/nn8000774 |
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| Author | Search for: Mahmoud, Khaled A.1; Search for: Hrapovic, Sabahudin1; Search for: Luong, John H. T.1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | environmental; protease; ferrocene-pepstatin inhibitor; carbon nanotubes; gold nanoparticles; biosensor |
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| Abstract | Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/ AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM. |
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| Publication date | 2008-04-01 |
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| In | |
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| Language | English |
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| NRC number | NRCC 49939 |
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| NPARC number | 12465493 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | dd837ce9-b257-4968-8029-a81522855e51 |
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| Record created | 2009-10-26 |
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| Record modified | 2020-04-15 |
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