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Picomolar detection of protease using peptide/single walled carbon nanotube/gold nanoparticle-modified electrode

Par Conseil national de recherches du Canada

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DOITrouver le DOI : https://doi.org/10.1021/nn8000774
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
FormatTexte, Article
Sujetenvironmental; protease; ferrocene-pepstatin inhibitor; carbon nanotubes; gold nanoparticles; biosensor
Résumé
Picomolar electrochemical detection of human immunodeficiency virus type-1 protease (HIV-1 PR) using ferrocene (Fc)-pepstatin-modified surfaces has been presented. Gold electrode surface was modified with gold nanoparticles (AuNP) or thiolated single walled carbon nanotubes/gold nanoparticles (SWCNT/AuNP). Thiol-terminated Fc-pepstatin was then self-assembled on such surfaces as confirmed by Raman spectroscopy and scanning electron microscope. The interaction between the Fc-pepstatin-modified substrates and HIV-1 PR was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both electrode materials showed enhanced electrochemical responses to increasing concentrations of HIV-1 PR with shifting to higher potentials as well as decrease in the overall signal intensity. However, the sensing electrode modified with thiolated SWCNTs/ AuNPs showed remarkable detection sensitivity with an estimated detection limit of 0.8 pM.
Date de publication
Dans
  • ACS Nano 2, nº 5 (1 avril 2008) : 1051–1057.
Langueanglais
Numéro du CNRCNRCC 49939
Numéro NPARC12465493
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrementdd837ce9-b257-4968-8029-a81522855e51
Enregistrement créé2009-10-26
Enregistrement modifié2020-04-15
Date de modification :