| Download | - View final version: Ultrafast separation and analysis of monoclonal antibody aggregates using membrane chromatography (PDF, 684 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1021/acs.analchem.7b00580 |
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| Author | Search for: Madadkar, Pedram; Search for: Umatheva, Umatheny; Search for: Hale, Geoff; Search for: Durocher, Yves1; Search for: Ghosh, Raja |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Abstract | We discuss a method for rapid and cost-effective analysis of monoclonal antibody (mAb) aggregates. Hydrophobic interaction membrane chromatography, which was previously shown to be highly suitable for such separation and analysis, was used in a recently developed format referred to as laterally fed membrane chromatography (or LFMC). A stack of rectangular polyvinylidene fluoride (or PVDF) membranes having 0.22 μm pores housed within a modified analytical-scale LFMC device was used for analyzing aggregate types and content in different monoclonal antibody samples. High-resolution separations could be achieved in less than 1.5 min, this being faster than other currently available techniques such as size exclusion ultraperformance liquid chromatography (SE-UPLC). Moreover, the operating pressure was less than 200 kPa, which eliminated the need for an expensive high-pressure pump and chromatography system. The resolution obtained using the LFMC was comparable to that obtained using SE-UPLC. The effect of design variations such as change in dead volume and pillar size within the lateral channels within the LFMC device was also examined. |
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| Publication date | 2017-03-27 |
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| Publisher | American Chemical Society |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23003451 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | e03330f2-5fe7-4ebe-8beb-a810dc9a9544 |
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| Record created | 2018-06-28 |
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| Record modified | 2020-05-30 |
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