| Téléchargement | - Voir la version finale : Ultrafast separation and analysis of monoclonal antibody aggregates using membrane chromatography (PDF, 684 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1021/acs.analchem.7b00580 |
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| Auteur | Rechercher : Madadkar, Pedram; Rechercher : Umatheva, Umatheny; Rechercher : Hale, Geoff; Rechercher : Durocher, Yves1; Rechercher : Ghosh, Raja |
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| Affiliation | - Conseil national de recherches du Canada. Thérapeutique en santé humaine
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| Format | Texte, Article |
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| Résumé | We discuss a method for rapid and cost-effective analysis of monoclonal antibody (mAb) aggregates. Hydrophobic interaction membrane chromatography, which was previously shown to be highly suitable for such separation and analysis, was used in a recently developed format referred to as laterally fed membrane chromatography (or LFMC). A stack of rectangular polyvinylidene fluoride (or PVDF) membranes having 0.22 μm pores housed within a modified analytical-scale LFMC device was used for analyzing aggregate types and content in different monoclonal antibody samples. High-resolution separations could be achieved in less than 1.5 min, this being faster than other currently available techniques such as size exclusion ultraperformance liquid chromatography (SE-UPLC). Moreover, the operating pressure was less than 200 kPa, which eliminated the need for an expensive high-pressure pump and chromatography system. The resolution obtained using the LFMC was comparable to that obtained using SE-UPLC. The effect of design variations such as change in dead volume and pillar size within the lateral channels within the LFMC device was also examined. |
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| Date de publication | 2017-03-27 |
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| Maison d’édition | American Chemical Society |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23003451 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | e03330f2-5fe7-4ebe-8beb-a810dc9a9544 |
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| Enregistrement créé | 2018-06-28 |
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| Enregistrement modifié | 2020-05-30 |
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