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Chemical structure of the carbohydrate backbone of the lipopolysaccharide from Piscirickettsia salmonis

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.carres.2013.04.010
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Affiliation
  1. National Research Council of Canada
FormatText, Article
SubjectBackbone structures; Chemical degradation; LPS; Methylation analysis; MS; Piscirickettsia salmonis; Pseudaminic acid; Structural information; Alkylation; Chemical analysis; Mass spectrometry; Nuclear magnetic resonance; Structure (composition); Carbohydrates; arabinose derivative; carbohydrate derivative; formic acid derivative; lipid A; lipopolysaccharide; monosaccharide; oligosaccharide; polymer; carbohydrate analysis; carbon nuclear magnetic resonance; chemical structure; degradation; mass spectrometry; methylation; Piscirickettsia salmonis; piscirickettsiosis; proton nuclear magnetic resonance; Piscirickettsia salmonis; Salmonidae
Abstract
Elucidation of the carbohydrate backbone structure of the lipopolysaccharide (LPS) from Piscirickettsia salmonis, the etiological agent of the salmonid rickettsial septicemia, is described. Structural information was established by a combination of monosaccharide and methylation analyses of LPS, and by NMR and mass spectrometries of oligosaccharides obtained through the use of various chemical degradations of the native polymer. The following structure of the backbone sugars was determined on the basis of the combined data from these experiments: (Equation presented) The presence of two consecutive residues of diacetylated pseudaminic acid (Pse5,7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy- L-glycero-L-manno-non-2-ulosonic acid) in the LPS appears to be unique among polysaccharides containing this acidic sugar. Similarly, the presence of 4-aminoarabinose (Ara4N, 4-amino-4-deoxy-L-arabinopyranose) on O-4 of the α-GlcN1P of the lipid A moiety is a unique feature of this LPS. © 2013 Elsevier Ltd.
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In
  • Carbohydrate Research 378 (2013): 108–113.
LanguageEnglish
Peer reviewedYes
NPARC number21270601
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Record identifiere53126ff-01ee-4fca-af74-1275b6eaf4c5
Record created2014-02-17
Record modified2020-04-22
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