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Chemical structure of the carbohydrate backbone of the lipopolysaccharide from Piscirickettsia salmonis

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.carres.2013.04.010
AuteurRechercher : 1; Rechercher : ; Rechercher :
Affiliation
  1. Conseil national de recherches du Canada
FormatTexte, Article
SujetBackbone structures; Chemical degradation; LPS; Methylation analysis; MS; Piscirickettsia salmonis; Pseudaminic acid; Structural information; Alkylation; Chemical analysis; Mass spectrometry; Nuclear magnetic resonance; Structure (composition); Carbohydrates; arabinose derivative; carbohydrate derivative; formic acid derivative; lipid A; lipopolysaccharide; monosaccharide; oligosaccharide; polymer; carbohydrate analysis; carbon nuclear magnetic resonance; chemical structure; degradation; mass spectrometry; methylation; Piscirickettsia salmonis; piscirickettsiosis; proton nuclear magnetic resonance; Piscirickettsia salmonis; Salmonidae
Résumé
Elucidation of the carbohydrate backbone structure of the lipopolysaccharide (LPS) from Piscirickettsia salmonis, the etiological agent of the salmonid rickettsial septicemia, is described. Structural information was established by a combination of monosaccharide and methylation analyses of LPS, and by NMR and mass spectrometries of oligosaccharides obtained through the use of various chemical degradations of the native polymer. The following structure of the backbone sugars was determined on the basis of the combined data from these experiments: (Equation presented) The presence of two consecutive residues of diacetylated pseudaminic acid (Pse5,7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy- L-glycero-L-manno-non-2-ulosonic acid) in the LPS appears to be unique among polysaccharides containing this acidic sugar. Similarly, the presence of 4-aminoarabinose (Ara4N, 4-amino-4-deoxy-L-arabinopyranose) on O-4 of the α-GlcN1P of the lipid A moiety is a unique feature of this LPS. © 2013 Elsevier Ltd.
Date de publication
Dans
  • Carbohydrate Research 378 (2013) : 108–113.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC21270601
Exporter la noticeExporter en format RIS
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Identificateur de l’enregistremente53126ff-01ee-4fca-af74-1275b6eaf4c5
Enregistrement créé2014-02-17
Enregistrement modifié2020-04-22
Date de modification :