Somatic embryo maturation from long-term suspension cultures of white spruce (Picea glauca)

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1007/BF02632280
Author	Search for: Dunstan, David I.¹; Search for: Bethune, Terry D.¹; Search for: Bock, Cheryl A.¹
Affiliation	National Research Council of Canada. NRC Plant Biotechnology Institute
Format	Text, Article
Subject	long-term suspension cultures; white spruce; somatic embryo
Abstract	The production of cotyledonary somatic embryos of white spruce from cultures grown long-term as suspensions was investigated. We report the effects of removal of 2,4-dichlorophenoxyacetic acid (2,4-D) from the maintenance medium (ordinarily containing both 2,4-D and benzyl adenine) before (±)-ABA-stimulated maturation. In particular the use of a 1-wk culture period without 2,4-D was found to improve the production of normal-looking cotyledonary somatic embryos. Using high performance liquid chromatography analyses of culture supernatants, it was determined that this affect was not related to altered ABA metabolism. Germination of cotyledonary somatic embryos from cultures pretreated by the 1-wk culture period without 2,4-D was improved compared with similar embryos from cultures that had not been pretreated.
Publication date	1993-07
Publisher	Springer Nature
In	In Vitro Cellular & Developmental Biology - Plant 29, no. 3 (July 1993): 109–112.
Language	English
Peer reviewed	Yes
Identifier	BF02632280 NRCC-35894
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Record identifier	e62de6cd-eb15-4be0-9f57-55fa1167d21a
Record created	2023-11-06
Record modified	2023-11-06

Date modified:: 2025-11-09