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Somatic embryo maturation from long-term suspension cultures of white spruce (Picea glauca)

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/BF02632280
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
Sujetlong-term suspension cultures; white spruce; somatic embryo
Résumé
The production of cotyledonary somatic embryos of white spruce from cultures grown long-term as suspensions was investigated. We report the effects of removal of 2,4-dichlorophenoxyacetic acid (2,4-D) from the maintenance medium (ordinarily containing both 2,4-D and benzyl adenine) before (±)-ABA-stimulated maturation. In particular the use of a 1-wk culture period without 2,4-D was found to improve the production of normal-looking cotyledonary somatic embryos. Using high performance liquid chromatography analyses of culture supernatants, it was determined that this affect was not related to altered ABA metabolism. Germination of cotyledonary somatic embryos from cultures pretreated by the 1-wk culture period without 2,4-D was improved compared with similar embryos from cultures that had not been pretreated.
Date de publication
Maison d’éditionSpringer Nature
Dans
  • In Vitro Cellular & Developmental Biology - Plant 29, nº 3 (juillet 1993) : 109–112.
Langueanglais
Publications évaluées par des pairsOui
IdentificateurBF02632280
NRCC-35894
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Identificateur de l’enregistremente62de6cd-eb15-4be0-9f57-55fa1167d21a
Enregistrement créé2023-11-06
Enregistrement modifié2023-11-06
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