| DOI | Resolve DOI: https://doi.org/10.1016/j.cryobiol.2011.04.009 |
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| Author | Search for: Ding, Fuhong; Search for: Lall, Santosh P.1 ; Search for: Li, Jun; Search for: Lei, Jilin; Search for: Rommens, Melissa; Search for: Milley, Joyce E.1 |
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| Name affiliation | - National Research Council Canada. NRC Institute for Marine Biosciences
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| Format | Text |
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| Type | Article |
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| Journal title | Cryobiology |
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| ISSN | 0011-2240 |
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| Volume | 63 |
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| Issue | 1 |
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| Pages | 56–60; # of pages: 5 |
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| Subject | Atlantic halibut; Hippoglossus hippoglossus, L.; cryopreservation; sperm; fertilization; motility |
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| Abstract | Development of Atlantic halibut (Hippoglossus hippoglossus) aquaculture will be enhanced with cryopreservation of halibut sperm by ensuring a reliable supply of sperm of desired quality and quantity. To assist in its commercial application, the cryopreservation of large volumes of halibut sperm was investigated. Three cryoprotectants were compared: dimethyl sulfoxide (DMSO), polyethylene glycol (PG) and glycerol (GLY) at two concentrations (10% or 15%). Two salt solutions, Hanks’ balanced salt solution (HBSS) and 0.1 M KHCO3 with 0.125 M sucrose solution (KS) were tested as diluents. Both factors were examined in 1.6 mL volumes. A cryopreservation volume of 4 mL and a low dilution ratio (1:1) were examined separately. Based on motility and fertilization rate, 10% and 15% DMSO diluted with HBSS or KS solution proved to be effective extenders with mean fertilization rates ranging from 52.2 ± 27.2% to 65.8 ± 26.1%; none of which were significantly different from that of the control. Four other extenders, 10% PG or 10% GLY with HBSS or KS, resulted in significantly lower fertilization rates. Use of a 4 mL cryopreservation volume did not exhibit a significant effect on fertilization rate or motility of post-thawed sperm compared to a 1.6 mL volume (P > 0.05); while the use of a dilution ratio of one part sperm with three parts cryopreservation solution (1:3 v/v with sperm concentration of 0.51 ± 0.11 × 1010 cells/ml) had a significantly better preservation effect than using a ratio of 1:1 with sperm concentration of 1.02 ± 0.21 × 1010 cells/ml (P < 0.05). From these results, an optimized protocol for the cryopreservation of Atlantic halibut sperm using a volume as large as 4 mL has been established. |
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| Publication date | 2011-04-28 |
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| Publisher | Elsevier B.V. |
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| Terms of use | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | 42752
1715 |
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| NPARC number | 3538228 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction |
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Record identifier | e7f9913a-ec29-4a2a-958b-16419bf4f188 | | Record created | 2009-03-01 |
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| Record modified | 2016-08-19 |
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