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Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.cryobiol.2011.04.009
AuteurRechercher : ; Rechercher : 1; Rechercher : ; Rechercher : ; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
FormatTexte, Article
SujetAtlantic halibut; Hippoglossus hippoglossus, L.; cryopreservation; sperm; fertilization; motility
Résumé
Development of Atlantic halibut (Hippoglossus hippoglossus) aquaculture will be enhanced with cryopreservation of halibut sperm by ensuring a reliable supply of sperm of desired quality and quantity. To assist in its commercial application, the cryopreservation of large volumes of halibut sperm was investigated. Three cryoprotectants were compared: dimethyl sulfoxide (DMSO), polyethylene glycol (PG) and glycerol (GLY) at two concentrations (10% or 15%). Two salt solutions, Hanks’ balanced salt solution (HBSS) and 0.1 M KHCO3 with 0.125 M sucrose solution (KS) were tested as diluents. Both factors were examined in 1.6 mL volumes. A cryopreservation volume of 4 mL and a low dilution ratio (1:1) were examined separately. Based on motility and fertilization rate, 10% and 15% DMSO diluted with HBSS or KS solution proved to be effective extenders with mean fertilization rates ranging from 52.2 ± 27.2% to 65.8 ± 26.1%; none of which were significantly different from that of the control. Four other extenders, 10% PG or 10% GLY with HBSS or KS, resulted in significantly lower fertilization rates. Use of a 4 mL cryopreservation volume did not exhibit a significant effect on fertilization rate or motility of post-thawed sperm compared to a 1.6 mL volume (P > 0.05); while the use of a dilution ratio of one part sperm with three parts cryopreservation solution (1:3 v/v with sperm concentration of 0.51 ± 0.11 × 1010 cells/ml) had a significantly better preservation effect than using a ratio of 1:1 with sperm concentration of 1.02 ± 0.21 × 1010 cells/ml (P < 0.05). From these results, an optimized protocol for the cryopreservation of Atlantic halibut sperm using a volume as large as 4 mL has been established.
Date de publication
Dans
  • Cryobiology 63, nº 1 : 56–60.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 42752
1715
Numéro NPARC3538228
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistremente7f9913a-ec29-4a2a-958b-16419bf4f188
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-21
Date de modification :