| DOI | Trouver le DOI : https://doi.org/10.1016/0304-4211(78)90070-6 |
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| Auteur | Rechercher : Huber, J.; Rechercher : Constabel, F.1; Rechercher : Gamborg, O. L1 |
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| Affiliation | - Conseil national de recherches du Canada. Laboratoire régional des prairies
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| Format | Texte, Article |
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| Résumé | A method was developed to determine the cell number of cell aggregates and embryos. The method was employed to study the rate of cell proliferation and frequency of embryo formation in a cell suspension culture of anise (Pimpinella anisum L.). The efficiency of embryogenesis was affected by the initial cell concentration (population density). In addition, a close relationship existed between the rate of cell division in cell aggregates and the efficiency of embryo-genesis. Maximum embryogenic efficiency and rate of cell division occurred in cultures with an initial population density of 10⁵ cells/ml. Cell proliferation in creased several-fold during the transition from the “heart-stage” to the “torpedo-stage”. This burst of cell division was preceded by a transient rise of the average cell volume. |
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| Date de publication | 1978-08 |
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| Maison d’édition | Elsevier |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | 0304421178900706 |
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| Numéro du CNRC | NRCC-16682 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 0b976244-60e7-4e79-b88f-c9e2a3081d96 |
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| Enregistrement créé | 2023-07-06 |
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| Enregistrement modifié | 2023-07-11 |
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