Téléchargement | - Voir le manuscrit accepté : Fabrication and performance of a photonic-microfluidic integrated device (PDF, 934 Kio)
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DOI | Trouver le DOI : https://doi.org/10.3390/mi3010062 |
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Auteur | Rechercher : Watts, Benjamin R.; Rechercher : Kowpak, Thomas; Rechercher : Zhang, Zhiyi1; Rechercher : Xu, Chang-Qing; Rechercher : Zhu, Shiping; Rechercher : Cao, Xudong; Rechercher : Lin, Min |
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Affiliation | - Conseil national de recherches du Canada. Institut des sciences des microstructures du CNRC
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Format | Texte, Article |
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Sujet | SU-8; PDMS; microfludics; optical waveguide; integration; devices |
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Résumé | Fabrication and performance of a functional photonic-microfluidic flow cytometer is demonstrated. The devices are fabricated on a Pyrex substrate by photolithographically patterning the microchannels and optics in a SU-8 layer that is sealed via a poly(dimethylsiloxane) (PDMS) layer through a unique chemical bonding method. The resulting devices eliminate the free-space excitation optics through integration of microlenses onto the chip to mimic conventional cytometry excitation. Devices with beam waists of 6 μm and 12 μm in fluorescent detection and counting tests using 2.5 and 6 μm beads-show CVs of 9%–13% and 23% for the two devices, respectively. These results are within the expectations for a conventional cytometer (5%–15%) and demonstrate the ability to integrate the photonic components for excitation onto the chip and the ability to maintain the level of reliable detection. |
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Date de publication | 2012-02-15 |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 19542538 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | 32a693ad-5082-4b37-a68b-06dc4d535456 |
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Enregistrement créé | 2012-02-29 |
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Enregistrement modifié | 2020-04-21 |
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