Host genetic requirements for DNA release of lactococcal phage TP901 ‐1

Par Conseil national de recherches du Canada

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DOI	Trouver le DOI : https://doi.org/10.1111/1751-7915.14156
Auteur	Rechercher : Ruiz‐Cruz, Sofía Identifiant ORCID : https://orcid.org/0000-0001-8968-5701; Rechercher : Erazo Garzon, Andrea; Rechercher : Kelleher, Philip; Rechercher : Bottacini, Francesca; Rechercher : Breum, Solvej Østergaard; Rechercher : Neve, Horst; Rechercher : Heller, Knut J.; Rechercher : Vogensen, Finn K.; Rechercher : Palussière, Simon; Rechercher : Courtin, Pascal; Rechercher : Chapot‐Chartier, Marie‐Pierre; Rechercher : Vinogradov, Evgeny¹; Rechercher : Sadovskaya, Irina; Rechercher : Mahony, Jennifer Identifiant ORCID : https://orcid.org/0000-0001-5846-6303; Rechercher : van Sinderen, Douwe Identifiant ORCID : https://orcid.org/0000-0003-1823-7957
Affiliation	Conseil national de recherches du Canada. Thérapeutique en santé humaine
Bailleur de fonds	Rechercher : Science Foundation Ireland
Format	Texte, Article
Résumé	The first step in phage infection is the recognition of, and adsorption to, a receptor located on the host cell surface. This reversible host adsorption step is commonly followed by an irreversible event, which involves phage DNA delivery or release into the bacterial cytoplasm. The molecular components that trigger this latter event are unknown for most phages of Gram-positive bacteria. In the current study, we present a comparative genome analysis of three mutants of Lactococcus cremoris 3107, which are resistant to the P335 group phage TP901-1 due to mutations that affect TP901-1 DNA release. Through genetic complementation and phage infection assays, a predicted lactococcal three-component glycosylation system (TGS) was shown to be required for TP901-1 infection. Major cell wall saccharidic components were analysed, but no differences were found. However, heterologous gene expression experiments indicate that this TGS is involved in the glucosylation of a cell envelope-associated component that triggers TP901-1 DNA release. To date, a saccharide modification has not been implicated in the DNA delivery process of a Gram-positive infecting phage.
Date de publication	2022-10-19
Maison d’édition	Wiley Open Access Applied Microbiology International
Licence	Creative Commons, Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/deed.fr
Dans	Microbial Biotechnology 15, nº 12 (19 octobre 2022) : 2875–2889.
Langue	anglais
Publications évaluées par des pairs	Oui
Exporter la notice	Exporter en format RIS
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Identificateur de l’enregistrement	c2293f3c-9df7-461d-ae3f-911e46083142
Enregistrement créé	2024-01-26
Enregistrement modifié	2024-01-26

Date de modification :: 2024-06-30